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Journal: Gastroenterology
Article Title: A Gut-Restricted Liver X Receptor Agonist Ameliorates Liver Injury in Experimental Short Bowel Syndrome
doi: 10.1053/j.gastro.2025.12.015
Figure Lengend Snippet: Aggravated IFALD following the deficiency of intestinal ApoA1 and HDL-C after SBR. ( A–D ) WT male ( filled circles ) or female ( open circles ) mice underwent sham or SBR. Starting 3 weeks later, mice received daily vehicle or WUSTL0717 (30 mg/kg, PO) for 7 weeks, followed by analysis (n = 5–9/group). ( A ) Heatmap of cholesterol efflux–related transcripts in the duodenum and post-anastomosis ileum from male mice (n = 3–4/group). ( B ) qRT-PCR analysis of Apoa1 transcripts in the duodenum, post-anastomosis ileum, and liver (n = 4–9/group). ( C and D ) Portal venous HDL-C ( C ) and ApoA1 ( D ) levels from males (plasma) and females (serum) (n = 5–9/group). ( E and F ) Correlation of portal venous plasma HDL-C and ApoA1 levels with liver collagen area ( , upper ). Each dot represents a matched individual from ( C ) and ( D ). ( G–M ) Apoa1 ΔIEC mice underwent SBR and were euthanized 11 weeks later. Dark dots represent males and light dots represent females (males, n = 7/group; females: n = 6–7/group). ( G and H ) Portal venous serum HDL-C and ApoA1 levels. ( I ) Systemic (inferior vena cava) serum ALT levels. ( J ) Liver Col1a1 transcript levels analyzed by qRT-PCR. ( K ) Sirius Red–stained liver sections ( scale bar , 200 μ m) with quantification from 5 to 6 areas per liver, 1 image per mouse (males, n = 7/group; females: n = 6–7/group). ( L and M ) Correlation of portal venous serum HDL-C and ApoA1 levels with liver collagen area. Each dot represents a matched individual from ( K )–( M ). Statistical evaluations were done using unpaired Student t test ( C , G–K ), 1-way analysis of variance with Tukey’s honestly significant difference ( B , D ), or Pearson correlation ( E , F , L , and M ).
Article Snippet: HDL-cholesterol (HDL-C) levels were measured using the
Techniques: Quantitative RT-PCR, Clinical Proteomics, Staining